Peptide Library Design Tools

Enter Protein Sequence

Amino Acid	3 letter code	1 letter code
Alanine	Ala	A
Cysteine	Cys	C
Aspartic acid	Asp	D
Glutamic acid	Glu	E
Phenylalanine	Phe	F
Glycine	Gly	G
Histidine	His	H
Isoleucine	Ile	I
Lysine	Lys	K
Arginine	Arg	R
Leucine	Leu	L
Methionine	Met	M
Asparagine	Asn	N
Proline	Pro	P
Glutamine	Gln	Q
Serine	Ser	S
Threonine	Thr	T
Valine	Val	V
Tryptophan	Trp	W
Tyrosine	Tyr	Y

• Overlapping Peptide Library

  The length of the input sequence should be greater than 10.

  Set Overlapping Peptides Design Parameters:

  Peptide Length (5-25)

  Amino Acid Overlap

  Overlapping Peptide Library

  Overlapping Peptide Library can be used in epitope mapping. The Peptide library generation process is defined by two parameters, peptide length and offset number. The offset number reflects the degree of overlapping. Careful selections of these two parameters are important to achieve optimum balance between data value and the experiment cost. As a general guideline, shorter peptides are easier to synthesize but they have less chances for multiple epitope hits. Greater degree of overlapping (small offset number) gives more chances of multiple epitope hits. Both above stated cases increase the number of peptides to be synthesized for the peptide library. Read More »

• Alanine Scanning Library

  The length of the input sequence should be between 6 and 200 inclusively.

  Alanine Scanning Library

  Alanine, the smallest chiral natural amino acid is used to substitute non-alanine residues at each position of the original peptide. The Alanine Scanning Library can be used to determine the relative importance of each substituted residues toward the original peptide's functionality since knocked off key residues reflect diminished peptide activity. Read More »

• Truncation Library

  The length of the input sequence should be between 10 and 200 inclusively.

  N-terminal Truncation

  C-terminal Truncation

  Truncation Library

  Truncation Library can be used to identify the shortest amino acid sequence needed for the peptide activity. The truncation process is carried out via a systemic reduction of resides from each flank of the original peptide. With the knowledge of the positions of key residues elucidated through Alanine Scanning Library, the construction of the truncation library could also be centered around these key amino acid residues. Read More »

• Positional Scanning Library

  The length of the input sequence should be between 10 and 200 inclusively.

  Please specify residue position or positions

  (please use ';' between adjacent position input)

  Positional Scanning Library

  Positional Scanning Library is one of the key methods for sequence optimization. Achieved by substituting selected amino acid residues by all other natural amino acids one at a time, it has the capability to locate potential more favorable residue(s) at specified position(s) for enhanced peptide activity. Read More »

• Random Library

  The length of the input sequence should be between 10 and 200 inclusively.

  Please specify random residue positions:

  (The number of positions N is between 2-3 ,
  The number of random combinations for N=2 is 400,
  and that for N=3 is 8000+400+400+400=9200)
  [ please use ';' between adjacent position input ],

  Random library

  Used in sequence optimization, Random Library is similar to positional scanning library. It's constructed by substituting selected positions on the original peptide randomly and simultaneously with all other natural amino acids in a shot gun approach with a purpose to elucidate potential alternatives for enhanced peptide activity. Read More »

• Scrambled Library

  The length of the input sequence should be between 10 and 200 inclusively.

  Please specify the library size (number of peptides within the library, maximum 50) :
  

  Scrambled Library

  Scramble Library is constructed by carrying out permutation on the original peptide's sequence. It has the potential to give all possible alternatives and offers and represents the highest degree of variability for peptide library. Read More »

• T Cell Truncated Library

  Input the peptide length

  Note: for most T cell epitope mapping applications, choose 11-mers as starting points for your peptide pools. The design tool will automatically generate 11-, 10-, 9- and 8-mer peptides. For other peptide lengths (ex. n-mer), n-, n-1, n-2 and n-3-mers will be generated.

  T-cell truncated library:

  T cell epitope identification is a critical part of designing highly specific immunotherapies. The T cell truncated library spans sequentially truncated sequences across a peptide sequence to identify important epitopes. Once identified, these sequences can be used to guide the development of targeted drugs or engineer specialized T cells for T cell therapy. Read More »